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The ammonia solution is transferred into a PTFE closed vial and is dried under a nitrogen stream.
Water was added in small volumes under permanent stirring in a tightly closed vial to avoid evaporation.
A simple and fast method for the TMS derivatization of phenolic acids in a closed vial using microwave irradiation followed by GC/MS analysis is proposed.
The extracted E. linza L. essential oil (EEO) was then kept in a tightly closed vial at 4 °C until further use.
Then, the seeded substrate was placed upside down into the solution contained in a closed vial at 90°C for 3 h to grow the nanorods.
The resulting solution is then exposed to MW at 300 W for 40 s in a closed vial and 20 ml of water is added.
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The use of three small volume PTFE closed vials (6-mL capacity) placed inside a conventional microwave vessel allows to drastically diminishing the volume of acid needed for digestion.
A multiple microwave-assisted digestion procedure using small PTFE closed vials (6 mL capacity) inserted into conventional microwave digestion vessels has been developed as a fast, efficient and clean methodology for multielemental analysis of human breast biopsies by inductively coupled plasma optical emission spectrometry.
After cooling, the coated strips were stored in separate, dry, clean, and closed vials.
Into 20 mL urine sample, 10 mL concentrated hydrochloric acid (37 %) was added and the mixture was heated in properly closed vials for 12 h at 110 °C to cleave aromatic amine adducts.
In this medium, bacteria were grown in hermetically closed Balch vials under anaerobic conditions at ~2 kl × tungsten lamp) and at ~25 °C till appearance of visually perceptible red suspension (equal approx. 107 cells/ml).
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