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For example, a needle must move forwards to pierce tissue or the jaw of a clamp must close to obtain a sample [ 32].
The only other transcriptomic study of Cx. pipiens f. molestus to date found that the relationship between species was sufficiently close to obtain reliable mapping results [ 26].
Both SNPs were sufficiently close to obtain phase information from the RNA-seq 45 bp PE reads (using novoPhase, see Materials and Methods) and these data confirmed that the same haplotype was over-represented at both SNPs.
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