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Intriguingly, the eukaryotic chaperonin TRiC (also called CCT) uses a built-in lid to close the chamber, whereas prokaryotic chaperonins use a detachable lid.
Close the chamber door; close the chimney one-half to three-quarters of the way; adjust to maintain the temperature inside the smoker between 225 and 275 degrees.
But members of the Freedom Caucus, seeing how close the chamber is to getting a vote on a Democratic-preferred DACA measure, is using their leverage on the farm bill to secure a vote on the Goodlatte bill to, in their minds, effectively kill the DACA discharge petition and reset the clock on when GOP moderates and Democrats could get a vote on the issue.
A 22 mm × 22 mm No. 1 coverslip (VWR) was placed on top of the stacks to close the chamber.
Bring chamber of freeze-fracture machine to atmospheric pressure, remove any condensation on the cold table using liquid Freon, rapidly insert frozen specimens onto the cold table, close the chamber door, and evacuate.
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He then closes the chamber, raises the temperature inside to several hundred degrees Celsius, and flows in silane and germane gases, which will provide the ingredients for the silicon-germanium nanowires.
Placed cleaned grids into the chamber of the glow-discharge equipment and closed the chamber.
Gas samples were taken from the chamber atmosphere at 0 20 40 min after closing the chamber using pre-evacuated 12-ml glass vials (Labco, Crewe, UK).
The bump structure, which acts as a backflow stopper by closing the chamber with the deflected membrane by positive pressure, inhibits an undesirable backflow toward the inlet and effectively transfers kinetic energy only toward the nozzle (Fig. 2b).
Especially in the range of lower pressure, the closer the chamber pressure is to the ultimate pressure of the vacuum pump, the more serious the asymmetry is, which coincides with the theoretical analysis.
In group 4 (topical), the thawed freeze-dried grafts were placed in the chamber and 8 μL (4 μg zoledronic acid) of experimental solution was pipetted to the graft just before closing the chamber with the screw cap.
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