With extreme dense genotype maps, it may even become possible to clone traits by haplotype-based in silico mapping [ 25], but to achieve this, it is estimated that complete sequences of over 50 strains are needed [ 29].
A number of large insert libraries, the foundation for most physical maps, have been developed for the Brassica A and C genome species and have been exploited to facilitate map-based gene cloning for traits of interest and to provide insights into the evolutionary mechanisms that have formed these complex genomes [ 29- 32, 35- 39].
These results revealed that Pi-Zn homeostasis interaction may vary even within the same plant species, which pave the way for a genetic study for cloning quantitative trait loci (QTL /gene(s) governing these traits.
While positional cloning is appropriate for cloning any trait that can be genetically mapped, there are situations for which a positional cloning approach may not be the best option.
These physical maps are a useful resource for comparative positional cloning of traits in cichlid fishes.
The resources developed herein can aid map-based cloning of traits of interest and the sequencing of the D genome of hexaploid wheat.
Genetic linkage mapping has proven to be very useful for analyzing quantitative trait loci (QTL), tagging and cloning genes controlling desirable agronomic traits and studying genome organization and evolution.
Unfortunately, few natural allelic variants have been cloned for drought related traits — progress cloning QTL, the use of RNA-sequencing methods for evaluating gene expression responses to soil drying, and improved methodology for exploring complex multivariate data all hold promise for moving the field forward.
Cloning QTL affecting complex traits has been a major challenge to plant geneticists and molecular biologists since the classical strategy using map-based cloning of QTL is extremely troublesome and time-consuming.
Cloning QTL affecting complex traits has been a major challenge to plant geneticists and molecular biologists since the classical strategy using map-based cloning is extremely troublesome and time-consuming.
