Sentence examples for cloning systems from inspiring English sources

Because of the limitations of the TA and Gateway cloning systems, most laboratories still use classic restriction enzyme cloning.

The use of cloning systems to produce homologous tandem repeats rather than the use of endogenous multidomain proteins has facilitated these developments.

The P. vivax ron4 gene's entire encoding sequence (2657 bp) was also used for observing how both vectors might behave when large-sized fragments were cloned into them; low recombinant colony frequency was found for both cloning systems: 50% (39 60 95% CI) cloning efficiency was estimated by pELMO and 30% (21 40 95% CI) by pGEM-T Easy vector (Fig. 3).

Cloning systems are characterised by allowing PCR product incorporation (Bernard 1996) into a circular plasmid; this can be further propagated to obtain an appropriate number of DNA copies whose integrity can be verified by sequencing and may be cryopreserved for future use (Gruber et al. 2008).

Such site-specific recombination based cloning systems have been successfully employed for building a number of clone sets [18], [20] [22], [40] [42].

In summary, the cloning strategy described here, that we call 'Golden Gate' cloning, combines the convenience and efficiency of currently used recombination-based cloning systems with unique insert precision.

An in vitro baculovirus cloning system has been developed for direct cloning of foreign DNA into baculovirus genomes.

Products were cloned using the TOPO TA cloning system following instructions of the manufacturer (Invitrogen).

All other ORFs were cloned using the Gateway® cloning system into the pDESTsmg vector [18].

A comprehensive gene collection for S. oneidensis was constructed using the lambda recombinase (Gateway) cloning system.

The pYUB1049 vector has been also converted to a Gateway® cloning system compatible vector, pDESTsmg [18].