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In the past it is only possible through lengthy and burdensome cloning procedures.
With recent advances, some scientists think safe and efficient cloning procedures will emerge in five to 10 years.
These manipulations typically involve cloning procedures where enzymes amplify, cut and ligate DNA in vitro.
He generated significant controversy by devising human cloning procedures as another avenue in treating infertility.
"Cloning procedures are more simple than we first thought," said Dr. Lannett Edwards, who led the effort here with her husband and colleague, Dr. Neal Schrick.
Standard molecular biology methods were used for all cloning procedures.
Standard molecular biology techniques were used for all cloning procedures (Sambrook et al. 1989).
Oligonucleotide sequences used for the cloning procedures are listed in Table 1.
E. coli DH5α (Transgen, Beijing, China) was used for gene cloning procedures.
E. coli Trans1-T1 (Transgen, Beijing, China) was used for cloning procedures.
However, some restriction sites existing on the pGreen backbone might interfere with further cloning procedures of the TuMV infectious clone.
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