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Another group subsequently reported cloning of the precursor of human PACAP38 and the human PACAP gene [17].
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Cloning of the amyloid precursor protein pioneered the investigation of Aβ aggregation in vitro[ 11].
Of course, we should acknowledge that the fine-scale cloning of the sRNA precursors is time consuming and laborious.
The function-first approach is based on bioassays, receptor-binding assays and genetic analysis, while the peptide-first approach is based on cDNA cloning of precursor, using neuropeptide precursor processing enzymes, peptidomics, etc. (7).
MGAH22 is a human/mouse chimeric IgG1 anti-HER2 antibody based on mouse clone 4D5, the precursor of trastuzumab [ 23] with an engineered Fc domain (MGFc0264) similar to the previously described Fc variant 18 [ 25], except that the V305I mutation was replaced by L235V to reduce CD32B binding.
Sinha, S. et al. Purification and cloning of amyloid precursor protein β-secretase from human brain.
We have isolated and characterized a full length cDNA clone encoding the precursor to the small subunit of wheat ribulose 1,5 bisphosphate carboxylase.
Several studies involving molecular cloning of biosynthetic precursor-encoding cDNAs from skin or skin secretions have revealed that these exhibit highly-conserved domain architectures with an unusually high degree of conserved nucleotide and resultant amino acid sequences within the signal peptides.
To verify the database prediction we sequenced the genomic clones of the Sly-MIR395 precursors.
Molecular cloning of the cDNA for ligatin.
If the sRNA precursor was experimentally cloned by using fine-scale methods such as RACE (rapid amplification of cDNA ends), the transcription boundary of the precursor-coding locus could be defined.
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