Sentence examples for cloning methodology from inspiring English sources

Using the services of Evrogen [ 50], full length cDNA was constructed using the Smart cDNA cloning methodology [ 51] and normalized using a duplex-specific nuclease [ 50].

Briefly, the service utilized the SMART cDNA cloning methodology to generate a full length cDNA pool [ 51], which was normalized using a duplex-specific nuclease [ 52].

Viral particles were pseudotyped with the vesicular stomatitis virus G-protein encoded in the previously described pMD2.G plasmid [ 24]. cDNAs encoding the 2 + 3-10+1N4R isoforms of human WT Tau and mutant P301L Tau were first cloned into the Gateway Entry pCR8/GW/TOPO vector (Invitrogen) using TOPO TA cloning methodology.

Based on Gateway cloning methodology, we have engineered a destination vector that directs expression of enhanced green fluorescent protein (EGFP -tagged protEGFP -taggedcell surface via a glycosylproteinsidylinositol tatl.

The GPIV promoter (wildtype) was cloned and each of the eight SNPs was introduced by infusion cloning methodology.

The pathway-specific TAR capture vector was constructed using InFusion cloning methodology (Clontech).

The system is compatible with multiple cloning methodologies, and has been demonstrated to produce baculovirus with equal or better titer and protein productivity than the currently available systems.

To mitigate this risk, we have developed a strategy that reduces the rate of fragment mis-assembly and is compatible with a variety of cloning methodologies.

Until recently, metatranscriptomics has been limited to microarray technology and random cloning methodologies.

The claMP Tag is similar in size to small chelating agents, but it bears the added advantage that it can be inserted inline with the protein sequence using standard cloning methodologies, allowing precise control of its placement.

So to reduce such costs Celesti et al. [93] proposed a Composed Image Cloning (CIC) methodology and focuses on the dynamic VM allocation.