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The growth and branching processes, performed by the so-called Tom Thumb algorithm, lead thus to the configuration and cloning mechanisms of the systems.
Constructs were generated to express pHluorin2 (Mahon, 2011) under the control of the β-actin and myosin6b (myo6b) promoter in a Tol2 transposon backbone (Kwan et al., 2007) using standard Gateway cloning mechanisms (Walhout et al., 2000).
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The proposed multi-way cloning mechanism is shown in Figure 4A.
This result suggested that the CPEC cloning mechanism was highly specific and did not favor carry-over of concatemers, if any, into the final clones.
Because genomic coordinates are not available for all loci and some genetically mapped loci are not cloned, a mechanism for estimating the genomic location of such loci is needed for, e.g. researchers walking to genes.
Hwang, in what is billed as an exclusive interview, now tells South Korea's Dong-A Ilbo newspaper that he, Mitalipov, and Xiaochun Xu, CEO of Boyalife Group of Wuxi, China, have agreed to jointly work on the mechanisms of cloning.
Sex outcompetes cloning by two complementary mechanisms.
Although several QTLs have been fine mapped, none have yet been cloned, so the mechanisms underlying the QTLs reviewed in this paper are still unknown.
Yet, new channels are being cloned and new mechanisms are being characterized every year and there is still huge uncharted territory to discover.
Through genetic mapping, molecular cloning and mechanism characterization of the zebrafish mutant cas002, we reveal a novel connection between ribosomal dysfunction and excessive autophagy in the regulation of hematopoietic stem/progenitor cells (HSPCs).
Therefore, map-based cloning of Im should be conducted for cloning this important gene, elucidating its molecular mechanism and further genetic manipulation to improve fiber fineness and maturity.
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