In other cases, researchers create genomic or complementary DNA (cDNA) libraries to identify interesting genes or to provide cloned material for sequencing.
Of course, while McGrady's website is by no means winning any design awards (and has clone material written all over it), or pushing the envelope technologically, it gets the job done, and, perhaps more importantly, it does so in a way that doesn't stink of brand shilling.
We created T5-lacO-lacO-gfp versions of our vectors (pBAV1K-T5-gfp, pLZ12-T5-gfp, pIMBB-T5-gfp, and pQE30-T5-gfp) in parallel, using overlap extension PCR cloning (Materials and Methods).
We used unique clone material derived from fully-grown Norway spruce trees with either high or low susceptibility to Heterobasidion spp. as measured in a field trial [ 7].
Dr. Primi cloned the material and then found it in the blood of other AIDS patients but not in otherwise healthy people in Italy.
Kelch motif BnACBPs were cloned from material grown in a semi-winter type area in China, they were the subject to further function analysis; but also, different lines SNP might exist as the material used for the sequencing of B. napus is not the same.
Thus, methylcytosines could be identified by sequencing of bisulfite PCR clones (Materials and Methods).
Using the standard approach, CsCl-purified H. akashiwo CCMP452 cpDNA was cloned into pUC18 plasmids and sequenced by the conventional shotgun cloning described in the Materials and Methods.
After cloning as described in Materials and Methods, the CHD-W and CHD-Z genes of S. c. hoya were sequenced and submitted to Genbank with accession nos.
Because our procedures respect the most stringent criteria of authenticity (independent extractions/amplifications, cloning and sequencing; see Materials and methods), we are confident that the ancient DNA sequences reported here are authentic and we used them in phylogenetic analyses.
