Many have done so, building funny online clones, incorporating Tetris elements, Dr. Who, the doge meme, 3D, and, of course, Flappy Bird itself.
All clones incorporating a PCR product were sequenced (SeqWright, Houston, TX).
In situ hybridization probes for the chicken genes derived from one clone incorporated into each contig were used.
The total set of expression clones incorporated a variety of promoters, solubility tags, and affinity tags.
The number of clones incorporated into wrong contigs represented only a small fraction of the total.
These findings, except for the clones incorporated into the 3AS contigs, are consistent with the assumption that most of the misincorporated clones were clones from chromosomes contaminating the chromosome or chromosome-arm specific libraries.
During fingerprint map construction BAC clones with extremes of insert size and number of restriction fragments were excluded from the analysis to screen out potentially mixed wells [ 21], thus clones incorporated into the map are unlikely to be heavily contaminated.
The majority of the clones incorporated on the microarray were derived from the BoMP cDNA library generated from bovine myeloid cells subjected to various stimuli, including over 900 sequences unique to the library.
As Facebook has already tried to clone or incorporate features from many existing startups (Facebook Questions, anyone?), this comes as no surprise.
We simplified the first recombineering step by incorporating gateway cloning sequence into our modified P[acman]-KO vector.
The design incorporates several cloning cores in order to be compatible with as many donor sequences as possible.
