Sentence examples for cloning design from inspiring English sources

VM participated in the cloning design.

SnapGene software (from GSL Biotech, Chicago, IL; available at www.snapgene.com) was used for molecular cloning design.

SWPC performed molecular cloning, design of plasmids, expression and purification of proteins, and design and running LC-MS assays.

This blunderbuss approach fails to distinguish between potentially valuable therapeutic cloning, designed to produce stem cells for human therapy, and the more questionable reproductive cloning, designed to produce a human baby.

The full-length EnStef gene was amplified using the rEnStefFWD (5′-CATAcata/tgATGCAAATGGTTGGAGGAATT-3′) and rEnStefREV (5′-GATAc/tcgagTCAAAAATATTCCAAGATATCGTC-3′) expression primers with NdeI/XhoI restriction sites for directional cloning (designed based on the sequence obtained after 5′ RACE PCR).

PR extracted total RNA, isolated polygalacturonase cDNAs, performed cloning, designed gene specific primers and performed preliminary RT-PCR expression studies.

AABV participated in the study design, cloning, designed and performed Arabidopsis transformation, histochemical assays, fluorimetric assays, manuscript writing, data interpretation and revision.

Primer pairs for cloning designed with PRIMER3 (http://frodo.wi.mit.edu/cgi-bin/primer3/primer3.cgi) are listed in Additional file 3: Primers used in this study.

"CloneAssistant 1.0" makes gene clone design much easier, and it can be freely downloaded from http://bis.zju.edu.cn/clone.

This data is necessarily heterogeneous in nature, ranging from materials and methods, construct and clone design, protein expression and purification to small molecule screening and protein structure determination information.

Among the 800 BAC clones, 17 spots were omitted from analysis because of collation with the latest genomic data and re-evaluation of clone design.