Sentence examples for cloning construct from inspiring English sources

It combines, within a single database software product, the ability to carry out comparative sequence alignments (Alignment Viewer ) that facilitates interactive protein construct design with virtual cloning (Construct Design Module ), followed by codon engineering of novel synthetic gene sequences that are optimized for protein expression in various recombinant systems (Gene Design Module ).

The integrity of the cloned construct was checked by nucleotide sequencing.

On 5 April, the sequence of the cloned construct was verified.

The final cloned construct contains the initiator Met codon and the 67 terminal amino acids (from residue 837 to 903) of human mt LeuRS.

An infectious FAdV-9 genomic clone, constructed by homologous recombination in Escherichia coli, was used for engineering of recombinant viruses.

The first full-length infectious cDNA clone of equine arteritis virus (EAV) was developed in 1996 and was also the first full-length infectious cDNA clone constructed from a member of the order Nidovirales.

Even successful flavivirus infectious cDNA clones constructed in low-copy number plasmids remain deleterious to E. coli, leading to slow growth and poor DNA yields [5].

Molecular poliovirus clones constructed to express two VPgs delete one of the two copies, and the polyprotein harboring two VPgs underwent aberrant processing [13], [14].

Mission™ shRNA clones constructed within the lentivirus plasmidvector pLKO.1-Puro were purchased from Sigma Aldrich (Milan, Italy).

Two libraries of BAC clones constructed from the genomic DNA of Misuzudaizu and William 82 were used.

We present an extensive expressed gene catalog for a commercially grown E. grandis × E. urophylla hybrid clone constructed using only Illumina mRNA-Seq technology and de novo assembly.