A cloning approach using restriction endonucleases LguI and/or Eco81I enabled a step-by-step continuous ligation of two fragments in vector pQE-30-LE.
However, the method is compared not with mixed seamless image cloning but with only seamless image cloning approach proposed in [1].
However, constrained PDE-based method in [15] and Poisson cloning approach in [6, 8] generate visually annoying effects as seen in Figure 8e,f.
Using the serological expression cloning approach we also identified bacterial antigens that are involved in these immune reactions.
With the aim of identifying the antigens of commensal gut bacteria that elicit systemic response in FMF we adopted a serological expression cloning approach.
It is very unlikely that we missed an entire plasmid with our shotgun cloning approach.
This is because cloning approach might exclude underexpressed miRNAs in a particular investigated tissue [ 35, 36].
Taken together, the cloning approach led to the identification of 31 miRNAs among the 152 non-redundant sRNAs.
This necessitates a highly efficient cDNA cloning approach which can make long eukaryotic transcripts available for functional studies.
It was predicted by our WGS and RNA-seq results, and validated by our shot-gun cloning approach.
An RT-PCR based cloning approach in combination with RACE was initially used to define the range of CYP4 genes expressed in C. gibbosum.
