Sentence examples for clones without the from inspiring English sources
Our aim was to develop a traceless approach to efficiently disrupt KRT5 alleles using TALENs displaying unbiased monoallelic disruption events and devise a strategy that allows for subsequent screening and isolation of correctly modified keratinocyte clones without the need for selection markers.
Aphids are parthenogenetic, capable of birthing clones without the hassle of sex.
Likewise, recovering clones without the insert's sequence coincides with numerous cases where plasmid vectors, having positive selection systems, have presented several recombinant clones lacking the insert (Ma et al. 2014; Pierce et al. 1992).
We have developed a fast and efficient Mu in vitro transposition-based procedure to construct targeting vectors directly from BAC clones without the need for prior subcloning.
In our hands, cloning and sequencing of 17 randomly selected cDNA clones yielded 9 clones without the 8 bp insertion (Genbank accession FJ621493), thus coding for the predicted full length protein, while 8 clones had the 8 bp insertion predicted to cause premature termination at codon 66.
The correctly targeted ES clones, without the Neo cassette, were injected into BALB/C blastocysts and implanted into pseudopregnant mice.
The use of Sags as therapeutic agents in T cell malignancies would have the advantage of deleting restricted T cell clones without causing the death of other normal cells.
APRILIA CAPONORD Something of a Suzuki V-Strom clone, without the value.
Dylan actually died of shame in 1964, shortly after he stopped pissing on warmongers' graves and became a sort of reverse-anachronistic John Cooper Clarke clone without the jokes.
Its descendants are liable to be indirect, thanks to the singularity that makes it a hit in the first place, and to the riskiness of exact cloning (since the authorized clone is almost always a letdown, and the clone without the "II" in its title seldom stands a chance).
A major strength of yeast display as a novel antibody discovery platform is the ability to characterize the binding properties, i.e., the affinity and epitope binding characteristics, of a clone without the need for subcloning, expression and purification of the scFv.
