Sentence examples for clones with two from inspiring English sources

Mutant clones were identified by the lack of GFP and the close proximity of twin spot clones with two copies of the GFP expression cassette.

The probability of obtaining plasmid clones with two or more transposon insertions is very low because, in the reaction conditions used, most of the target DNA fragments do not experience even a single transposon integration [14], [16].

From clones with two consecutive ESAG6 genes, the ESAG6 hypervariable region from the most telomeric ESAG6 gene, was reamplified from the largest PCR fragment by nested-PCR with primers: 5'-CACTAATGATCAGCTTTACG-3' and 5'-GACTCTTTTACACGTGAATC-3'.

The first, 3xGAS-lacZ [39], is located on the same chromosome arm as ept, such that mitotic recombination of a 3xGAS-lacZ,ept2,FRT80B chromosome produces clones of ept cells carrying two copies of the 3xGAS-lacZ transgene that can be compared to discs carrying control FRT80B clones with two copies of 3xGAS-lacZ.

Digestion of clones with two endonucleases (Eco130I and HindIII) yielded, on average, 30 products per clone.

The WWDYG motif was hit in 11 single mutant clones and in four clones with two amino acid changes (Table 2).

We describe here their affinity maturation by error-prone PCR and ribosome display yielding clones with zero to seven (average 2.5) amino acid substitutions in framework positions.

In a given round of CoS-MAGE with ten ssDNA oligonucleotides, the five nuclease knockout strain has on average 46% more alleles converted per clone, 200% more clones with five or more allele conversions, and 35% fewer clones without any allele conversions.

And that means they make shitty clones with zero prospect of gaining loyal fans.

RPS19 cDNA clones with three w.t.

In addition, there were many instances of clones with one to three identical SNPs, plus one to many additional SNPs unique to each clone.