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Reproducibility of fingerprinting techniques was assessed by repeatedly fingerprinting randomly selected clones (with more than 30 bands) and using Genoprofiler software [25].
The frequency of spontaneous labeling of clones with 200 to 400 cells was actually only 1.3×10−2 (2/144) and that of clones with more than 400 cells was 2×10−2 3/1444) (Table 3, first line, non-injected controls).
Individual clones with more than 50 cells were counted.
Finally, datasets of F2 clones with more than 25% of their genotypes invalid were rejected.
The clones with more than 50 cells were counted under an ordinary optical microscope.
A large number of clones with more than 15bp of repeat motifs were found among the markers developed.
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For example, the CMRSA2 "Other spa types" comprise 10%% of this clone with more than 40 unique spa types present in this category.
A 1 1 to 3 1 molar ratio of insert:vector yielded highly efficient cloning, with more than 2 × 10 colonies/ng vector being formed (Fig. 2b).
We therefore need to confirm this gene as a possible basis for AR in the Kan10 clone with more detailed genotypic/phenotypic associations.
In 1.4% (3 patients), there was a dominating clone with more than 75% BRAF V600E mutated cells, which can be regarded as a valid rationale for BRAF inhibitor treatment.
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