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Refolding was, however, efficiently prevented by combining denaturation with reduction of the intra-domain disulfide bridges, which created favourable conditions for selection of clones with improved stability.
Subsequent analysis of the ability of soluble xyloglucan to inhibit the binding of these XGBM displayed on phage to immobilized xyloglucan, identified three clones with improved properties.
The method enables quantitative screening of gene libraries to identify clones with improved activity and it also allows cells to be selected based not only on the overall activity but also on the specific activity of the enzyme.
Rare clones with improved solubility characteristics and able to form stable complexes with a co-expressed bait are isolated from the random library using a high-throughput, automated screening workflow based upon commonly accessible robotic systems.
Positive clones with improved thermostability.
Two mutations, K316N and D500N, were found in several clones with improved volumetric activity towards ABTS.
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Our findings demonstrate that RNAi technology combining animal cloning offers the possibility to produce TG animal with improved resistance to viral infection.
Comparative genomic hybridization (CGH) of metaphase spreads has a resolution of approximately 20 Mb, and array-based CGH with genomic or cDNA clones has improved this to <1 Mb.
Using a reporter protein downstream of the synthetic gene, allows the selection of clones with random mutations improving the expression or the folding of the protein of interest.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com