Exact(3)
This design creates tags that are specific for one in more than a million clones, which in theory allows single-clone analysis of the complete TCRβ repertoire on high-density microarrays.
In contrast to clones of cells expressing only GFP ectopically (Figure 4H H″), clones which in addition overexpressed ph formed smooth borders with neighboring wild-type cells and in some cases reorganized into a cyst structure clearly segregating from the rest of the disc (Figure 4J J″).
The disadvantage of using short 60-mer probes is the low hybridization signal, which in theory is a factor of about 2000 times lower than when using the much larger insert BAC clones, which in turn suffer from variable but high DNA repeat content that must be quenched by Cot1 (the dominant fraction of human repetitive DNA sequence -DNA in the hybridization mix.
Similar(57)
The results were also consistent with the contig assembly in which 7 of the 8 clones were assembled in a patch in ctg567 while the absence of the last BAC was due to failed HICF fingerprinting of the clone, which in turn excluded the clone from the framework physical map.
Regenerated clones, which appeared in 7 14 days, were picked and subcultured in the individual wells of a 24-well micro-plate containing 1 mL/well of semi-solid CYM medium (pH 6.5) supplemented with 20 µg mL−1 hygromycin.
This prevents the elimination of sensitive tumour clones which should, in theory, suppress the growth of therapy resistant clones in a competitive manner.
Among the 34 miRNA families mir159 showed the largest number of sequenced clones which are in agreement as miR159 is also the most abundant family in Arabidopsis[ 16].
Asexual reproduction, in which no gametes (sex cells) participate, produces genetically identical progeny (clones), which separate in larger animals (e.g., sea anemones).
Only clones which differed in migrating position were selected for sequencing.
For this, we used all 32 finished clones which map in the region.
We sequenced five of the 10 BAC clones, which ranged in length from 98 kb to 202 kb (GenBank Accessions: KF541650; KF541651; KF541652; KF541653; KF541655, Table 1), while the remaining five BAC clones overlapped the sequenced clones.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com