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However, expression analysis on MVA and MEP pathway genes suggest that the MEP pathway can be an alternate provider of IPP in mature rubber trees or in clones which do not produce a large amount of carotenoid [ 8].
It is assumed that grafting of olive trees provided a means to propagate clones which do not root easily, and increased the survival rate of trees, since newly grafted trees required less attention than cuttings [ 12].
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BT/HerR0.2 clones, which did not have up-regulated PPP1R1B in the microarray analysis (data not shown), were also analyzed in this experiment.
Additionally, some therapeutic targets may be present only in a few nondominant clones, which does cause unresponsiveness to the treatment.
Clones which did not grow in the selection medium or did not yield sufficient quantity or quality of plasmid DNA were discarded.
Clones which did not show a signal for target ORFs but the correct signal for the integration event were subjected to sequencing.
For these analyses, the clones which did not exceed a frequency of 10% in at least one sample of N ≥ 10, were pooled and labelled as "rare".
Of these, 50 RefSeq-linked iTSCs were further supported by cDNA clones, which did not overlap the RefSeq transcripts (Fig. 5E and F).
The average insert size of clones from the BAC library is 102.9 kb, based on a selection of n = 186 clones which doesn't include this BAC clone [ 19].
Interestingly, it was necessary to express the recombinant antigen in a mammalian system, as previous attempts to generate anti-CA IX antibodies from bacterially produced catalytic domain led to the isolation of antibody clones, which did not recognise the antigen in its native conformation (data not shown).
He was the chief executive of another company, Genetic Savings & Clone, which did extensive research on cloning dogs but concentrated on the commercial potential of cloning customers' cats, something it offered to do for $50,000 apiece.
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