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For all the experiments two myrAkt clones were tested.
After three rounds of panning, phage clones were tested for binding specificity to 8H3.
PAB clones were tested for the production of bacterial proteins after transformation of E. coli host cells and protein production in cell lysates.
Six independent clones were tested to ensure reproducibility.
2 positive clones were tested for SMAD1 expression with Western blotting analysis after Cre recombination.
In most cases, 2 independent clones were tested per each mutant.
Established T-cell clones were tested for reactivity to MAP or E. coli.
All three clones were tested for effects on growth rate and none were seen (Figure S4).
Progeny obtained from these six clones were tested by BrdU assay (Fig. S3).
Conditioned media from hybridoma clones were tested for antibody production and specificity.
The periplasmic extracts from individual clones were tested by indirect ELISA.
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