Sentence examples for clones were successfully from inspiring English sources

Positive BAC clones were successfully selected from each species, although the genome coverage of their BAC libraries is not as high as that of the AGI libraries.

Through Southern hybridization of BAC filters with the PCR product (156-bp) amplified from exon 3 of Hd6, candidate BAC clones were successfully screened from each BAC library.

Stably transfected clones were successfully established and expression of recombinant EGFP TPMT in homogeneous cell populations was demonstrated by flow cytometry, fluorescence microscopy and immunoblotting.

Sixteen sites with three or more clones were successfully located by this aerial survey approach, which increased the number of natural Japanese red maple sites of the database by 34% and the area of natural sites by 54%.

Thus, a total of 30,512 (82.7%) clones were successfully fingerprinted for use in contig assembly (Table 1).

Colonies displaying hESC morphology were expanded on MEFs and the clones were successfully maintained under feeder-free conditions using mTeSR media and Matrigel coated plates.

Of 379 clones isolated from the screen, 123 clones demonstrated ≥25% difference in mean YFP fluorescence and the gene trap genomic insertion site for 47 of the 123 clones was successfully determined by inverse PCR (supplementary material Table S1).

After removal of clones containing less than 20 fragments or greater than 220 fragments and empty vectors, 95,386 clones (88% of the original total), with an average of 114.2 fragments per clone, were successfully assigned a digitized fingerprint (Table 1) to use for assembly carried out using the FPC software [ 25].

The 3.3-kb TaEPSPS-7A1 clone was successfully recovered using buffer D and 5 % DMSO, and sequenced in both the forward and reverse directions (Table  1).

It is also clear that not all of the clones that were successfully transfected with a construct expressing an active integrase yielded recombinant products.

As shown in Fig.  1, all of the clones examined were successfully located at the expected positions on the corresponding chromosomes.