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The differences in the restriction pattern among the genomic clones were studied using PCR-RFLP.
O. Kuntze) clones were studied four consecutive years (2003/2004 2006/2007), in a large (9 ha) field experiment comprising of six drip irrigation treatments (labelled: I1 I6) and four clones (TRFCA PC81, AHP S15/10, BBK35 and BBT207) planted at a spacing of 1.20 m × 0.60 m at Kibena Tea Limited (KTL), Njombe in the Southern Tanzania in a situation of limited water availability.
At least three different independent clones were studied for each mutation.
Among those, two MT-LynWT and two control clones were studied in details and gave similar results.
A total of 210 clones were studied for methylation status of TRβ gene in head tissue of X. laevis (see Figure S1).
Specifically, the sampling intervals were adjusted to a single Daphnia generation (i.e. 14-30 days, depending on temperature) and the spatial distribution of taxa and clones were studied within individual water bodies.
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Differentiation of the clones was studied by transferring individual clusters onto laminin-coated surface, and allowing them to differentiate for one week.
The genetic variation among the genomic clones was studied in more detail using the Neighbor-Joining method, which resulted in four significantly different groups of genomic γ-gliadin sequences.
Interestingly in three independent experiments the final culture density, as determined by OD600 after a 16 hr induction, reached a value reproducibly characteristic to the clone being studied ranging from 0.8 for C2 to 1.7 for N4.
The induction of HPRT-mutations and survival of Chinese hamster cells (line B11ii-FAF28, clone 431) were studied after irradiation by 4He and 12C-ions of various LET (20 – 360 keV/μm), produced by the U-200 heavy ion accelerator.
To minimize clonal variations, at least three clones per orientation were studied.
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