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We were a few scant years out of the boy band/Limp Bizkit era, and Pearl Jam clones were still proliferating, each one worse than the last (Stone Temple Pilots > Creed > Nickelback).
Dg O86 clones positively marked by mCD8GFP expression did not show a polarity phenotype under either condition, but false damage-induced clones were still observed (Fig. 5A C).
These findings indicate that although there was a 29% over-estimation of genes affecting fermentation in the second screen (where optical density was measured), 71% of the clones were still identified as affecting fermentation.
However, the rescue was not complete, as UAS -dpn RNAi; E spl Xp UAS -N intra mutant clones were still larger than the controls (Fig. 6E) and the ectopic expression of Cut and Wg was not completely eliminated (Fig. 6).
Nevertheless, clones with noise levels similar to control clones were still present, as was previously observed with the original protocol (Freed et al. 2008) and might be explained by aggregation with nonfluorescent cells in the sorting process.
As the authors state in the article: "One week after YAP induction… many of these clones were still composed of single cells, indicating that cell division is not necessary for the initiation of dedifferentiation"; "YAP activation does not simply lead to hyperplastic response as no enrichment was found when compared to a gene signature derived from livers recovering from partial hepatectomy".
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Now, even as the TV family bid their last farewell, the hunt for their clones is still going on.
Westhusin explains, however, that dog clones are still a long way off.
Only clones near the point of misassembly are affected; the vast majority of clones are still locally ordered correctly with respect to their neighbors.
However, 60 of the 64 clones are still F+ clones.
The proportion of empty clones was still about 2%.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com