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Clones were sequenced using an ABI 3730 automated sequencer.
Positive clones were sequenced with primers T7/SP6 by a DNA sequencer (ABI 3730).
The positive clones were sequenced and analyzed.
Of these, 1248 PCR positive clones were sequenced and analyzed.
About 60 positive clones were sequenced in each library.
Recombinant plasmid pLL3.7-HmLZ-IRES2-EGFP positive clones were sequenced.
In general, a total of 45 50 clones were sequenced for each embryo.
From the library for archaeal amoA genes, 24 randomly drawn clones were sequenced.
The recombinant plasmid was verified by PCR, and positive clones were sequenced.
Twenty-four unique clones were sequenced and preliminarily identified based on sequence homology.
A total of 624 clones were sequenced; 516 had repeats and 94 were duplicates.
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