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Sixty interacting clones were retained (Additional file 4).
After further validation (see Methods), 24 clones were retained for analysis.
These 76 clones were retained for a second analysis with triplicate biological cultures for each strain.
Eight specific clones were retained (Additional file 2), four (50%) representing Drosophila Rab6 (dRab6).
A total of 269,712 intensity readings from both channels and corresponding to 8,538 unique clones were retained for the analysis.
When the screen was performed using ROF7 as bait, a total of 191 specifically interacting clones were retained from the mammalian cDNA library.
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The purified fosmid clones are retained at Washington University and used as templates for finishing reactions by using student-designed primers to obtain additional sequence data.
A total of 77,237 BAC end sequences (BES) with a mean size of 671 bp were obtained from sequencing both ends of 44,544 BAC clones, and were retained after quality check [ 38].
After a preliminary screening for PRRSV susceptibility, 3 CHO clones (IC5, ID9 and IF3) and 2 PK15 clones (IXH7 and IXA3) were retained for further analysis.
Clones displaying both activities were retained for further analysis.
In cases where the ORF was not present or only partially cloned due to internal deletions or mispriming events or where introns were retained, the cloning was repeated.
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