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On the other hand, baz PFC mutant clones were reported to show strong multilayering (Abdelilah-Seyfried et al., 2003).
Identification numbers for sdY-containing clones were reported on at the Plant and Animal Genome Conference in 2013 (Palibroda et al. 2013).
Only unique clones were reported; uniqueness was assessed by random PCR generated SNPs, some of which were the result of less than complete bisulfite mutagenesis.
Like the present study, 61% of clones were found to contain SSRs in the study of He and colleagues [ 20], 56% clones had SSRs in the study of Gimenes and colleagues [ 18] and 43.7% clones were reported to contain SSRs by Wang and colleagues [ 24].
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The best five mutants in terms of higher activity were sequenced and the corresponding mutations of the selected clones are reported in Table 1.
The seven selected clones are reported in Supplementary Fig. 2.
The deduced AA sequences of the cDNA clones are reported in Additional file 7.
We found that each bas-1 'YK' clone, however, contained a different internal deletion (overall abnormality of these clones is reported at ~5% – J. Theirry-Meig, personal communication).
The simple, easy, and high-throughput PCR screening methods for identifying BAC clones are reported in many investigations [ 22, 32, 33, 37] using different pooling schemes.
The deduced amino acid sequences of the V-D-J regions of all 72 cDNA clones are reported in the Table 1 together with the corresponding TRBC genes, according to the tissue of origin.
The number of EST clones is reported to be proportional to the abundance of cognate transcripts in the tissue or cell type used to make the cDNA library and thus the EST distribution can provide a quantitative assessment of differential expression of a gene [ 17].
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