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Several clones were picked and propagated individually.
After 10 14 days EGFP-positive clones were picked and further cultured for characterization.
Individual clones were picked and the targeting locus (Dazl) was amplified followed by sequencing.
Individual clones were picked and DKOzero clone 15.1 was chosen for all further experiments.
After puromycin selection, 3 clones were picked and expanded.
Two weeks later, clones were picked and screened by RFLP and DNA sequencing.
For the phylogenetic analysis of bacteria, more than 100 clones were picked and sequenced.
After selection with puromycin, three different cell clones were picked and verified by PCR (Fig. 1B).
Positive clones were picked for mini plasmid preparation (QIAGEN, Hilden, Germany).
White clones were picked randomly and re-amplified using primer set M13F/M13R to screen positive clones.
After additional two weeks, G418-resistant clones were picked and verified by genomic PCR and DNA sequencing.
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