Sentence examples for clones were mixed from inspiring English sources

For the screen, thawed cells from the library (corresponding to 5×107 independent clones) were mixed with MATa strain cells (CG1945) transformed with the Engrailed bait plasmid, plated on complete medium for 5 h, and transferred onto Tryptophan/Leucine/Histidine deficient plates (DO-W-L-H) supplemented with 2.5 mM 3'-amino triazol (3AT).

For microscopy, cells of the 15,000 clones were mixed, grown in complex medium, and analyzed for fluorescence.

2 × 106 cells consisting of equal parts of either three ST-3 or mock clones were mixed with 50 μl matrigel and injected into the mammary fat pad.

For most mixes, the clones were divided into sets and all combinations of clones were mixed within that set.

T cell clones were mixed with naive splenocytes to obtain a total cell number of 1×106.

Purified positive clones were mixed with nonreactive phage clone (β-gal phage) as an internal negative control at a 1 100 ratio.

Glycerol stock (4 μl) of a BAC-containing E. coli clone was mixed with the Buffer D1 (4 μl) and incubated on ice for 5 minutes.

Fifty microliters of phage supernatant of each clone was mixed with 50 μL of 100 ng mL 1 parathion-methyl in 10% methanol PBS or pure dilution buffer.

It has been argued that this anomalous distribution of p-values is, in turn, a consequence of the own experimental design of the dataset, in particular the lack of biological replication and the way clone aliquots were mixed to produce each gene group with a given fold change56.

The phage clones (2×107 pfu) were mixed with 1 M of dextrose, galactose, mannose or N-Acetyl glucosamine in an eppendorf tube for 1 hr at RT. Then 5×106 C. albicans cells were added to the tube for interaction and the plaque assay was performed as described above.

Genomic DNA isolated from the placentas of three randomly chosen deceased cloned calves (SCNT) were mixed in equal amounts to generate a pooled sample as SCNT.