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The polyadenylation sites of the positive clones were mapped on the RNU1 locus.
To obtain a stem cell gene list (SGL), clones were mapped to their corresponding EntrezGene identifiers.
If unmapped clones were mapped on Gap region in genome, we expected the annotation profile of unmapped clones would be similar to that of mapped clones.
For some cDNA clones, the orientation in which the clone was mapped onto a chromosome and the chromosome on which the clones were mapped were not consistent among the assemblies.
The remaining sequences of the other 13 BAC clones were mapped independently over ST II with one BAC clone, breambac-141b24, being re-located on ST XVI instead of ST II.
BAC clones were mapped to the hg17 genome build.
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In this approach, a library of bacterial artificial chromosome (BAC) clones is mapped onto chromosomes by the use of molecular markers, the aim being to generate a clone-based physical map with a "minimum tiling path".
In contrast to the Tuzun approach, which discarded clones mapping to recent segmental duplications and considered only a single best placement for every clone-end on the genome, we use a local search algorithm to find an assignment of each clone to a genomic locus, where our confidence in a particular assignment grows if other clones are mapped nearby.
a. Two ends (≤ 300 kb apart) derived from the same BAC clone were mapped to the same chromosome with the same orientation.
For the current study, the peptide specificity of the 15HEL clone was mapped.
Each clone is mapped to a UniGene accession based on UniGene build # 162.
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