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Since over-expression experiments do not allow the modulation of kinase activity both in terms of timing and signal strength and, moreover, since the levels of transgene expression in selected clones were lower than in the heterozygous cells, the combination of these drawbacks might explain the incomplete rescue (Fig. 2A).
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PHB accumulation in all the mutant clones was lower than that of the wild type clone, suggesting that the wild-type PHB polymerase is highly optimized for PHB accumulation defined as the 'fitness landscape model' (Taguchi et al. 2001).
Despite this biological difference, the detectability of individual parasite clones was lower in P. vivax than in P. falciparum.
Although about 230 of 2400 unmapped clones were confirmed to map on the rice genome by other genome assemblies (Table 1), the proportion of highly-homologous unmapped clones was lower than that of mapped clones and the proportion of no-homologous unmapped clones was higher than that of mapped clones (Table S6).
Since the larger distances could be, in the case of MNase, due to the fact that the number of non-redundant clones was lower (165 vs 200; see Table 2), 165 randomly chosen apoptotic clones were investigated and distances were found to be comparable to those of MNase clones (5.4 Mb).
Interestingly, the virulence of P. ramosa clones was lower than that of isolates.
This experiment was also performed at 17°C, a temperature at which the Gal4 system is less active and therefore the levels of en over-expression in the clones are lower.
For channel catfish, the average number of blue (BamH1) and red (XhoI) fragments per clone were lower than the number of green (EcoRI) and yellow (XbaI) fragments.
HPRT mRNA levels in all TG-resistant clones were substantially lower than those observed in the Dox treated parental cells (Fig. 3).
Cisplatin IC50 values for both clones were 50% lower than in non- RAB25-expressing cells.
Furthermore, profound expansion of Vλ clones employing identical CDR3s were observed in the adult CD5+ B cells, the fetal cells, and the SLE repertoire, whereas the frequency of the Vλ clones were much lower in the adult CD5- B-cell population.
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