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Six positive clones were identified by four SSR markers and were end-sequenced (Table3).
Eight positive clones were identified by Southern blotting with 5′ probe and 3′ probe.
Positive clones were identified by the blue and white screening method.
Results: Twenty-one differentially expressed cDNA clones were identified between the 2 cell lines.
It is abundantly expressed in placenta, as numerous EST clones were identified.
Eleven clones were identified covering the Qbp1 locus (the locus between markers R1925 and G1318 on chromosome 3).
The other three clones were identified to have single-crossover at the upstream homologous arm (data not shown).
Among these 63 QTLs, positional candidate genes or BAC/PAC clones were identified in 50 QTLs by high-resolution mapping.
As shown in Fig. 1D, two clones were identified to have precise HR at both arms and no random integration.
Several correct clones were identified.
Cell clones were identified by LacZ or GFP staining.
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