Sentence examples for clones were first from inspiring English sources
The growing clones were first verified by PCR-amplicon sequencing then confirmed by Western blot analyses of Rho expression induced after addition of Ponasterone A (Sigma Aldrich ref: P3490) into the medium at 5 µg/ml.
The all-female clones were first discovered in 1995 by German pet traders and quickly became a popular addition to home aquariums.
Random recombinant plasmid clones were first selected from each library for Sanger sequencing.
Shotgun clones were first screened with primers used for BAC isolation.
The G418-positive ES clones were first screened by long-range PCR and then confirmed by Southern blot analysis.
The initial encounter with tumor derived antigens most likely occurs in the tumor-draining lymph nodes where specific clones were first found expanded.
The receptor(s) expression in the cell clones was first detected by dot-blot of cell lysates using commercial available antibodies and wild-type CHO cells lysates as negative basal staining.
In this protocol, additional steps are included in which low frequency clones are first clustered with high frequency clones, to correct for sequencing errors.
The presence of the different Psg genes within YAC, BAC and cosmid clones was first determined by PCR followed by hybridisation with oligonucleotides specific for individual Psg genes.
The pluripotency of these gene-corrected clones was first analyzed both by alkaline phosphatase (AP) staining and immunohistochemistry staining of different pluripotency genes.
In situ monitoring with such Tradescantia clones was first tried in 1974 around a nuclear power plant in Japan, then has been repeated until 1979 around more nuclear plants.
