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For micro-scale expression, clones were expressed in 96 deep well format (100 μl volume) overnight at 30°C with 0.1 mM IPTG using the pHOG 21 system.
Sixty-two cloneslones were expressed differentially between the control and 300 g conditions: the expression levels of 39 clones increased, whereas those of 23 clones decreased under hypergravity conditions.
These two clones were expressed, purified, and characterized.
About 41% of the clones were classified as constitutive, being expressed about equally in roots, fruit and aerial tissues, while 23% of the clones were expressed higher in roots than other tissues.
After electrotransformation, around 100 colonies from the selected library were isolated and soluble proteins of these clones were expressed using the nonsuppressor strain E.coli HB2151.
Twenty two annotated genes (24 clones) were expressed at significantly higher levels in SA72T0.
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Whole-mount in situ hybridization demonstrated that 17 of the clones are expressed in the kidney.
The numbers of cloning were expressed as mean±S.D. from at least three independent experiments.
The efficiency of cloning was expressed in a number of viable clones per plate.
HCV-LPs derived from the cDNA of the infectious clone H77 were expressed and purified as described previously [24] [24].
The four cloned ADH genes were expressed in E. coli Rosetta DE3.
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