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Exact(25)
For BLAST comparison, the sequences from uncultured clones were excluded.
These clones were excluded from further experiments.
Both chimeric and very short clones were excluded from further analysis.
Clones were flagged, if they were outside the range of two times the standard deviation; these flagged clones were excluded from later statistical analysis.
For the present analysis only clones associated with OTUs found in two clone libraries generated within days of the start of each experiment (31 July 2005 and 18 September 2005) were used and singleton clones were excluded (those forming a unique OTU with no other clones >97% sequence identity found in any of the four libraries).
Identical clones were excluded from the analysis as well.
Similar(35)
Mixed primary cultures which failed to be purified by a single filter cloning were excluded from the study (data not shown).
In cases when the number of samples for each clone became less than 3 due to the above filtering or some other bad conditions, the clone was excluded from calculation to secure further robustness.
Lastly, 163 clones that were considered as Questionable-clones (Q-clones) and were excluded from the physical map were added to the MTP, in order to assess these clones for possible use as bridge clones in the final assembly.
Single clone alterations were excluded from further analysis, ensuring that multiple, neighbouring clones spanned candidate regions.
Sequences with Phred-scores bellow 20, as well as identical ditags (which are likely to be the result of amplification or cloning artifacts) were excluded from further analysis.
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