Sentence examples for clones were engineered from inspiring English sources

Because clones were engineered to express different fluorescent markers, outgrowth of the dominant clone was easily assessed by fluorescent imaging and FACS analysis (Fig. 4).

Two variant clones of each enzyme were engineered to increase protein solubility and to facilitate purification, as reported for CYP2B enzymes from multiple species.

We screened a human B cell-derived scFv phage display library and identified a panel of BCMA-specific clones from which human CARs were engineered.

Two clones of portal vertex protein were engineered by attaching a Strep-tag II (WSHPQFER) to either the N-terminus or the C-terminus of each gp10 subunit.

Polyproteins (I69 8 and (I91ΔCys)2-I69-(I91ΔCys)2 were engineered by iterative cloning using BamHI, BglII and KpnI restriction sites27.

The vectors were engineered to facilitate cloning and shuffling of the functional modules with or without transcriptional terminators.

The spanning loops, site 6 of GFP, were engineered with RE cloning sites for inserting oligonucleotides corresponding to FcɛRI-binding sequence of human IgE.

PCR products containing wild-type and mutant pVHL19 coding sequences were cloned into BamHI/ XhoI sites that were engineered into another retroviral vector, pQCXIP (Clontech, Mountain View, CA).

To test the hypothesis that U18666A targets NPC1, we compared its ability to block the esterification of LDL-derived cholesterol in CHO-7 cells and in TR-4139 cells, a clone of CHO-7 cells that were engineered to stably express excess NPC1.

In the second approach, CR-EACs were engineered to express PIAS γCA clone.

Therefore, the human CD80 promoter was cloned and luciferase reporter constructs containing various promoter fragments were engineered.