Sentence examples for clones were digested from inspiring English sources

Positive entry clones were digested into two fragments using MluI and were purified using sodium acetate precipitation.

For RFLP analyses, purified plasmid DNA, of individual clones, were digested with NotI and NdeI.

Few additional clones were discerned with BsuR1 (HaeIII); therefore the remaining 384 clones were digested with AluI only.

First, the DNA from resistant clones were digested by PstI and probed with probe DR (an MfeI fragment of AC026683, position 98899-99822) locatedownstreamam of the targeted region.

The random regions of the clones were digested with BglII and XhoI and subcloned into the pET20 vector (Novagen) containing the N-terminal T7·tag sequence and the C-terminal His6 tag sequence.

The inserts of the first 96 clones were digested with the restriction enzymes BsuR1 (HaeIII) and AluI (Fermentas) overnight at 37°C with the appropriate buffer and 10 units of enzyme.

Extracted DNA from BAC clones was digested with NotI and the digestion products were subjected to pulsed-field gel electrophoresis (PFGE) as described previously [ 57]: 25 μl DNA from each clone was digested by NotI in a 30 μl reaction mix and loaded on PFGE.

DNA from antibiotic-resistant clones was digested with BamHI and subjected to Southern blot analysis.

Two micrograms of genomic DNA from P. falciparum NF54 [31] and Δmaebl clones was digested with EcoRv or NsiI.

DNA derived from individual BRI2tADan/+ ES clones was digested with BamHI, gel separated, blotted into a nylon membrane and hybridized with either the 5' or the 3'probe.

DNA derived from individual Y682G or T668A ES clones was digested with BamHI, gel separated, blotted into a nylon membrane and hybridized with the 3'probe.