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Expression clones were designed to allow exploration of possible fusion partners and investigate both enzymatic and chemical cleavage as means to liberate the target peptide.
MISSION shRNA clones were designed and developed by The RNAi Consortium (TRC) at the Broad Institute of MIT and Harvard University.
All ORF clones were designed based on the sequence information provided in the databases described above.
Fifty primer pairs for expression analysis of Ra-induced clones were designed based on the sequences of raphanusanin-induced ESTs using Primer3 software (see additional file 8).
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A humanization library of size 1.6×106 clones was designed for the murine anti-VEGF antibody A4.6.1 [27], [27], based on antibody structure modeling and the human germline sequence.
This first study of involving lymphodepletion and CD8+ T cell clones was designed to limit toxicity of lymphodepletion while balanced to promote homeostatic cytokines and reduce regulatory T cells.
These libraries differ from other gene-rich datasets in having larger insert sizes, and the MSLL clones are designed to provide reads localized to "epigenetic boundaries" where methylation begins or ends.
Primers for cDNA cloning were designed based on database-deposited partial sequences, which anneal to the untranslated region of XtClock, XtBmal1, XtCry1, XtCry2 and Xtβ2M genes (Table 1) or inside the ORF region of XtHprt1 and XrGusb genes.
The artificial microRNAs (amiRNAs) and the primers for subsequent cloning were designed according to the procedures and criteria on the website (http://wmd3.weigelworld.org/cgi-bin/webapp.cgi) [46] using the miR319a precursor-containing plasmid pRS300 as a template.
The gene-specific primers used for gene cloning were designed using Primer Premier 6 software (http://www.premierbiosoft.com/).
Primers for SCS cloning were designed from the 3' end and 5' untranslated region of contig 00661, which contained an entire ORF.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com