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For mouse Dlk1 and Meg9/Mirg, cDNA clones were described previously; however, our results increase our understanding of these genes.
GM13130 polyclonal cell line and GM13130 clones were described before (Gimelbrant et al., 2007).
The construction of normalized cDNA library and sequencing of cDNA clones were described previously [ 14].
The latter two types of Leu+ clones were described previously (Watanabe & Horiuchi 2005).
Human ARF1 (NM-001024228 -GFP NM-001024228 -GFP were described previously (Gill et al., 2010).
K562 clones were described with integrated copies of a BCL11A-XL expressing vector.
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The antigen retrieval method details and primary antibody clones are described in Table 5.
The growth and preparation protocols for the clones are described in Additional file 1.
The methods used for isolation of positive clones are described in a previous report [ 41].
Initial characterization of these clones was described earlier [ 6, 8] and clone number 1623 was examined further in this study.
The direct PCR procedure for each regenerated clone was described previously (Yamagishi et al. 2013).
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