Sentence examples for clones were combined from inspiring English sources
To screen the library, clones were combined in a pooling strategy and specific loci were identified by PCR analysis.
These new clones were combined and treated as single individuals for final measurements.
The SSR, SNP, AFLP, and DArT genotyping data for 180 DMDD progeny clones were combined and screened for polymorphic markers.
The contents of two plates (192 clones) were combined for extractions using QIAprep Spin Miniprep kits (Qiagen, Venlo, The Netherlands).
The two clones were combined using PCR with the outermost 5′ and 3′ primers, RL1 and RL5.
When the data for all the clones were combined, the higher soil N level was reflected in the higher N content of the belowground biomass (Table 1).
The targeting hooks were placed in the promoter region of URA3 as shown in Figure 2. Genomic DNA and the linearized TAR cloning vector were combined with yeast spheroplasts and the His+Ura- transformants were selected on medium containing 5-FOA to identify clones with genomic DNA inserts.
For population dynamic analyses full VAX003 gp120 sequences (only one clone per patient) were combined with other full length, dated Thai gp120 sequences from the Los Alamos database as of January 2010 to generate final datasets of 343 CRfromAE (from 1990 to 2006) and 47 subtype B (from 1990 to 2003) isolates.
In each superpool, cultures from each plate (384 clones), row (24 clones × 20 plates), and column (16 clones × 20 plates) were combined, respectively, to make DNA for the second step screening.
In a 500 µL volume, 100 representations of each clone or 1e+11 phagemids were combined with 10 µL streptavidin coated M-280 magnetic beads (Dynal Biotech ASA, Oslo, Norway) on a rotisserie to pre-absorb background binders for 1 h in PBS+2% bovine serum albumin+0.05% Tween-20 (PBSBT).
The IL-17A capture complex was freshly made every time just prior to use as follows: 2 µL of a biotin-labeled CD45 antibody (clone HI30; Caltag/Invitrogen, CA) were combined in an eppendorf tube with 20 µL (0.5 mg/mL) of biotin-labeled IL-17A antibody (Ebiosciences) and mixed thoroughly.
