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Then genome sequencing and high throughput library screening projects delivered data on the genome sequence of many organisms, and clones were collected and made available to researchers in a previously unparalleled quantity.
Cells from different clones were collected to prepare genomic DNA with a genomic DNA extraction kit (Aidlab, China).
The pre-sorted libraries were then amplified by growth in liquid cultures before expression of TEVp and the substrate libraries were induced, and the fluorescent clones were collected.
TCR β-CDR3 mRNA sequences of human T-cell clones were collected from the public database of NCBI at NIH. Vβ-, Jβ-, Dβ-segments were identified using the V-QUEST algorithm from the international ImMunoGeneTics information system [13].
For immunostaining, third instar larvae containing single-neuron clones were collected, dissected, fixed in 3.7% formaldehyde in for 15 min, and permeabilized in 0.1% Triton X-100 in phosphate-buffered saline.
For immunostaining, third instar larvae containinig single neuron clones were collected, dissected, fixed in 3.7% formaldehyde for 15 minutes and permeabilized in 0.1% Triton X-100 in phosphate-buffered saline.
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This number may be an underestimate if FLcDNAs are not randomly cloned, but most of the genes should be included if more than 100 000 clones are collected from closely related species.
The fingerprint profiles for each BAC clone were collected by GeneMapper v3.7 (Applied Biosystems).
According to the affycomp help file, "The sequences of each spiked-in clone were collected and blasted against all HG-U133A target sequences.
The fingerprint profiles for each BAC clone were collected by GeneMapper v3.7 (ABI) and then converted to a data format suitable for FPC application via GenoProfiler v2.1.
Faecal samples used for %G+C fractioning and cloning were collected from ten donors (six females, four males), with an average age of 46.5 years that suffered from IBS according to the established Rome II criteria [ 17].
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