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Based on the frequencies of overlapping 20-mers for each BAC clone, the clones were categorized into low repetitive (0 40% repetitive), mid repetitive (40 70% repetitive), and high repetitive (70 100% repetitive) clones (Fig. 1a).
Annotation was assigned through the use of protein homology database using homologous sequences and clones were categorized as known or novel gene.
The rest of the uniquely mapped 1,375 clones were categorized in detail.
Clones were categorized as specific binders if they had a binding ratio of target:irrelevant ≥ 2.0, as described in Table 2 above.
(ii) clones were categorized by individual and by tissue type, e.g. hair (H) and blood (B), knowing from previous research [ 7] that hair may be more Numt-rich than blood.
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Overall, we cloned a total of 174 miRNAs from 363 cDNA clones which were categorized into 46 different miRNA species (Table 1), with miR-21, miR-24, miR-27a, and miR-205 being most abundant.
Methylation levels for each CpG site were indicated by the ratio of methylation positive clones to the total number of clones sequenced, and were categorized into three groups in cell lines: high (ratio > 0.5), low (0 < ratio < 0.5) and none (ratio = 0) (marked as dark, gray and white dots, respectively).
In addition, 2 clones were found to have deletions at their gene segments but not at the complementarity-determining regions (CDRs) and were categorized as partial clones.
Clones that had a binding ratio of ≥ 2.0 over streptavidin (SA) background were categorized as binders.
The cells were categorized into four groups.
Both countries were categorized as "D" nations.
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