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The cumulative frequencies of common T-cell clones were calculated as frequencies from GPLs/EGC.
The mean diversity and mean p-distance of amino acid mutations in each genomic fragment of 10 independent clones were calculated (Table 6).
Similarity matrices among the sequences of the clones were calculated to identify the operational taxonomic units (OTUs) that were further used to estimate species richness (Chao-1) using the Web-based rarefaction calculator software (http://www2.biology.ualberta.ca./jbrzusto/rarefact.php)./jbrzusto/rarefact.php
Total primary clones were calculated from standard serial dilutions.
The distances between individual clones were calculated with the assistance of DnaSP 4.0 software [ 33].
Distances between the clones were calculated with the CLUSTAL W program [ 68].
Similar(51)
Next, the numbers of clones are calculated and cloning starts.
The percentage of Ampr/Kms clones was calculated for each case (n = 100).
(3) The proportion of male-producing clones was calculated for each clonal cross as the proportion of clones where males were observed among the viable F1 clones.
(4) The proportion of diapausing egg-producing clones was calculated for each clonal cross as the proportion of male-producing F1 clones that produced diapausing eggs (the result of successful selfing within the F1 clone), so taking into account that diapausing egg production requires the previous production of males.
SD of all X-chromosome clones was calculated for each hybridization experiment.
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