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In these clones, we observe no splitting of the cell cluster and the mutant lineage never uses the mediolateral antennal lobe tract.
Using three mir-510 independent clones we observe a two- to four-fold increase in migration and four- to five-fold increase in invasion across coated membranes.
In stage 9 follicles with Dl clones, we observe the persistence of Ncad expression, indicating that Ncad represents a second shared target for TGFβ and Notch signalling (supplementary material Fig. S5D).
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Indeed, in mosaic third larval instar wing discs containing large coldf02290 Minute+ clones, we observed that the cold mutant cells fail to accumulate the FasIII marker in their most apical part, unlike the surrounding wild type cells (Fig. 7A,A').
Employing immunofluorescence analysis of these SH-SY5Y clones, we observed that ectopic lipin-1α exhibited both nuclear and cytosolic localization, with a higher concentration in the nucleus (Fig. 6B).
In these clones, we observed a dramatic re-localization of GFP-Cindr.
Amongst these clones, we observed two different 5'-flanking sequences and three different 3'-flanking sequences.
In 4 5 day positively marked clones, we observed clonal sizes ranging from typically 2 11 cells (data not shown).
Finally, in the ARO-Pax8 clones, we observed a lower growth rate in comparison to ARO non transfected cells (Fig. 6).
When apoptosis was blocked in tubulin-labelled otd null clones, we observed a partial rescue of the cell loss phenotype in the adult brain (Fig. 2G I).
During the generation of doxorubicin resistant MCF-7 clones, we observed the presence of multiple nuclei, in the enlarged cytoplasm of cells.
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