Sentence examples for clones we next from inspiring English sources

In that changes in gene expression are ultimately responsible for abnormalities in clones, we next focused our analysis on the regions identified that mapped to promoters and were either hyper- or hypo-acetylated at H3K9.

Since the intergenic 79 bp segment between the polyA site and the Sac I site alone did not restore barrier function either in vivo or in L cell clones, we next asked whether the presence of the distal intergenic segment between the Sac I site and the Bam HI site alone was sufficient to restore barrier function.

Given the observed up-regulation of DKK1 in cisplatin-refractory NSCLC residual clones, we next analyzed basal mRNA DKK1 expression levels in NSCLC cell lines with the aim to reveal if there was a correlation between basal DKK1 expression level and cisplatin sensitivity (Fig.  4c).

To further characterize the performance of each clone, we next calculated the AUROC, and sensitivity and specificity given the optimal cutoff of the clones.

Therefore, using one of the most stringent criteria for demonstrating the quality of iPS clones [23], we next tested the frequency of their germ-line transmission and the production of viable F1 offspring.

To circumvent cloning bias issues, we next tested the ability of pyrosequencing technology [33] to (a) generate genome sequence from small amounts of phage DNA, and (b) allow de novo assembly of whole phage genomes.

We next cloned small hairpin RNAs (shRNAs) targeting MBP-1 (MBPsi-4) mRNA into a pRNAH1.1/neo plasmid vector (Genscript) under the control of the H1 promoter.

We next cloned FMR4 into a pcDNA3.1 vector with a CMV promoter and overexpressed FMR4 in HEK-293T cells, a pcDNA3.1 vector without the FMR4 insert was used as a control.

Since our spectral measurements were performed with a synthetic form of NvFP-7R predicted from genome sequence and EST data, we next cloned the endogenous gene and repeated the experiments.

We next cloned intronic genomic DNA sequences that flank mouse exons 1, -2a and -2b (including exons -2c and -2d) at their 5' end (Figure 7A,B; referred to as DNA fragments A I) as well as mouse exon -2a itself (Figure 7B, DNA fragment J, which contains the TF-binding cluster), into the pGL3 reporter vector to drive expression of firefly luciferase.

We next cloned, expressed, and purified LexA from reference strain PA01.