A common technique to study gene knockout phenotypes is to examine single cell clones and we hypothesized that for a cell line which is dependent on a certain gene, it should be difficult, if not impossible, to generate clonal homozygous knockouts for the gene of interest.
Because the RIR junction between the ArntbHLH and C/EBP LZ is not an optimal sequence for promoting the seamless α-helical structure shown in the crystal structure, as it was introduced for cloning purposes, we hypothesized that although this hybrid would bind to the E-box, its activity could be lower than that of the native Max bHLHZ.
We hypothesized that clone circulation within a team would be easier to detect in large team or team with the highest carriage.
We hypothesized that cloning of the putative adhesin gene cluster coding for the putative subunit protein, putative usher and chaperone proteins and putative adhesin, which together are believed to be responsible for expression of fimbrial structures, when successfully cloned in an afimbriate E. coli strain would enable the expression of EA/1 fimbriae visible under the electron microscope.
We hypothesized that tumor clones that constitutively overexpress this pathway are preferentially selected by the host microenvironment due to a resistance to STAT1-dependent cytotoxicity and demonstrate increased metastatic ability combined with increased resistance to genotoxic stress.
On the basis of the differential marker expression profiles, we hypothesized that NP-R clones represent a NP stem cell progenitor-like phenotype, whereas the NP-nR clones might have been immortalized at a more advanced differentiation or maturation stage.
We hypothesized that the two clones would respond differently to a change in these climatic parameters, due to their genetically inherent differences in adaptation to distinct climatic origins.
We hypothesized that the two clones would respond differently to temperature and CO2 due to their inherent differences in ecological adaptation.
If clone 5F7 indeed regulates the OLIG genes, we hypothesized that they may interact in chromosomes.
When it emerged that she was heterozygous for G6PD A- we hypothesized that her expanded PNH clones may be expressing only the G6PD A- protein, which would have explained the observation of the emergency physician.
