Sentence examples for clones we generated from inspiring English sources

To investigate the behaviour of boundary-respecting clones we generated a spatial model using mathematical tools.

To validate the various clones we generated specific HIF-1α expression constructs including full-length HIF-1α, HIF-1αP402R HIF-1αP402RR and HIF-1αP564R,P564R.

All the clones we generated in this study produced both type 1 and type 2 cytokines: IFN-γ, TNF-α, IL-13 and in some cases IL-10.

With an aim to identify FOXM1B-target genes involved in malignant transformation, we next questioned if the FOXM1B-transformed clones we generated in Fig. 4H may harbour 'signature' genomic instability loci associated with malignant transformation.

To improve the assembly for the Class II clones we generated additional sequencing reads.

More importantly, the clones we generated confirmed that CD44posCD24pos cells give rise to functionally heterogeneous progeny.

By inverting as previously the BP reaction between the P3-ccdB/CmR-P4-DONR cassette and the pSP72-B3-a-element-B4-bpFOG-B5-B1-Kozak-NLS-LacZ-stop-B2 expression clone, we generated the pSP72-R3-ccdB-CmR-R4-bpFOG-B5::B1-Kozak-NLS-LacZ-stop-B2 destination vector.

To check for unwanted effects caused by genes flanking Tfb1m in the BAC clone, we generated transgenic mice harboring a version of the BAC clone (BAC-KO) in which Tfb1m expression was silenced by deletion of exon 3.

Briefly, for each brain-derived cDNA clone, we generated digoxygenin-labelled sense and antisense riboprobes, performed in situ hybridization using high-stringency hybridization and wash conditions, and detected cellular labelling by immunohistochemical detection with alkaline phosphatase precipitation.

In line with these RNAi knockdown results, we were able to recover multicellular btd mutant INP clones that were comparable to wild-type INP clones while we generated btd mutant type II NB clones, indicating that btd mutant INPs were still able to divide multiple rounds like wild-type INPs and did not prematurely differentiate into GMCs.

Instead of a twin-clone analysis, we generated GFP marked wild type cells, since twin clone analysis was not reliable in testes GSCs (Fig. 4D,E).