We construct three types of read sets that consist of all reads that putatively overlap a region of the genome delineated by clone extent endpoints: For each clone we create a clone read set, which contains all the reads overlapping the clone (including reads from other clones) (Figure 6a).
For each clone Ci we create a contig set Bi that consists of all the contigs from a read set that is completely contained within the extent of the clone Ci.
To investigate whether this phenotype reflects a specific requirement for Gmd in FNG-dependent Notch signaling, or a more general requirement for Gmd in Notch signaling, we created clones of cells homozygous for Gmd.
It is unlikely that a mammoth could be cloned in the way we created Dolly the sheep, as has been proposed following the discovery of mammoth bones in northern Siberia.
To facilitate the mapping of candidate genes and to increase the density of markers available for positional cloning, we have created a radiation hybrid (RH) map of the zebrafish genome.
We created three clones for these developers, named after their usernames: jeresig, adam, and aakosh.
Furthermore we created control clones, conditionally expressing only EGFP (Co 5, Co#11), in order to exclude unspecific effects caused by EGFP expression or by doxycycline treatment.
To examine the effects of SRC-1 and CBP on breast cancer cell phenotype and gene expression, we created stable clones expressing these coactivators.
Thus, to investigate the consequences of inactivation of WASF3 in these prostate cancer cell lines, we created stable clones from PC3 and DU145 cells carrying shRNAs targeting WASF3.
To examine the potential role of VDAC1 in regulating extrinsic pathway death signaling in NSCLC cells, we created H460 clones, stably expressing shRNA targeted to VDAC1.
