Sentence examples for clones we constructed from inspiring English sources

Using the pMCEscan, which is a novel and stringent selection system used to create a few high protein-producing clones, we constructed engineered CHO strains that can be used for high-level production of foreign proteins by gene targeting.

To create stable clones, we constructed vector expressing luciferase protein (pENTR.Luc2.ON) and recombined it with the plko.dsRed2.DEST vector, thus creating the destination clone plko.dsRed2.Luc2.ON.

In order to systematically analyze genes expressed in developing castor seeds and to facilitate functional analysis of the cDNA clones, we constructed an oriented full-length cDNA library in a lambda vector that incorporated the Gateway cloning system.

For generation of stable clone, we constructed a plasmid DNA vector expressing a potent shRNA targeted to MBP-1 coding region or scrambled shRNA.

To reduce the risk of nonrandomness of clone coverage, we constructed seven paired-end libraries, with insert sizes of approximately 250 base pairs (bp), 300 bp, 500 bp, 2 kb, 9 kb, 10 kb, and 20 kb (Supporting Information, Table S1).

As two allelic forms (FRD359T/367M and W138Q/307S) were not available among the cloned envelope genes, we constructed them by exchange of restriction fragments (BsmI-NotI for FRD359T/367M and KpnI-XhoI for W138Q/307S).

To assess the autotrophic potential and the biosynthetic pathways involved in carbon fixation of the epibiotic community we constructed clone libraries for genes encoding for subunits of key enzymes of two carbon fixation pathways, RubisCO for the Calvin-Benson-Bassham (CBB) cycle and the ATP-dependent citrate lyase (ACL) for the reductive tricarboxylic acid (rTCA) cycle.

To study the exact roles of Dok6 in the cortex, we constructed knockdown clones of Dok6 (Dok6i) and nonsense controls (details are given in the Methods section).

To further characterize the P1 virus, we constructed two clones, one with a single copy of P1 virus genome and one with a two copy tandem repeat.

For the cloning of the Pi2-2 gene, we constructed a genomic BAC library of Jefferson with an average insert size of 140 kb.

For C. convolvulus, H. "sanjuanensis," and L. unguiculata, we constructed random clone libraries from the purified PCR product using the TOPO Shotgun Subcloning Kit from Invitrogen (for details, see Burger et al. 2007) and sequenced at the Office of Biotechnology DNA Facility of the Iowa State University.