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After the selection of best clones, we also evaluated the cloning techniques between 96 well plate cloning and ring cloning, single cell clones of ELec1 (Best transfected cells) from 96 well plates were imaged directly.
In addition, in all of those clones, we also detected another 7 single nucleotide polymorphisms (SNPs) in the D-loop region, 2 in the 12S rRNA gene, 2 in the 16S rRNA gene, 1 in the tRNA-R gene, and 23 in protein genes (Fig. 3D, Table S1).
In these clones, we also observe induction of endogenous en activity.
Through further analysis of individual paclitaxel resistant clones, we also identify potential modifiers of ABCB1-mediated resistance.
In addition to these clones, we also added 36 previously characterized cDNA clones as controls on the array (Tables 4 and 5).
In order to benchmark these clones, we also expressed the corresponding standard periplasmic scFv and scFv-AP formats from the pSANG10-3F and pSANG14-3F vectorespectivelyely in the Origami 2™ (DE3) strain.
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In an A. baumannii background (strain M9 from which blaADC was initially cloned), we also see a reduction of MIC values from >64 to 16 μg/mL for S02030.
By comparing the eleven clones, we could also measure the genetic variability (heritability) of these traits and their flexibility (see Materials and Methods, sections C and D).
All of the 11 otd −/− single cell clones we recovered also displayed a similar neuroanatomy.
In addition to the proliferation and cell death defects of scrib mutant clones, we have also observed that they cooperate with activated alleles of dRas1 (RasACT) RasACT or Notch (NACT) to repress pupal development and, throughout an extended 'giant larvae' phase of development, form massive and invasive tumours [ 2, 3].
DOI: http://dx.doi.org/10.7554/eLife.04437.007 To verify that the low frequency of Egfr f24 FSC clones was not due to a lower rate of clone induction, we also quantified the FSC clone frequencies in the control and experimental groups at 2 dpci, which is the earliest time at which GFP FSC clones can be detected.
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